• Karina Dafne Martinez University of Buenos Aires
Keywords: Soy Protein, Hydrolysate, Polysaccharides, Air–water interface, Surface pressure, Dynamic measurements.


The objective of the work was to study the influence of soy protein concentration, and the hydrolysis on kinetic adsorption to the air-water interface with the effect of polysaccharides addition. As starting material a sample of commercial soy protein isolate was used (SP) and hydrolysate (H) at 2, 10-2 and 10-3%wt/wt of concentrations, was produced by an enzymatic reaction. The degree of hydrolysis was 2%. The polysaccharides (PS) used at 0.25%wt/wt of final concentration were  hydroxypropylmethylcellulose (E4M) and lamda carrageenan (lC). The dynamic surface pressure of films were evaluated with a drop tensiometer. We determined the kinetic parameters of adsorption to the air-water interface: the diffusion (Kd), penetration (Kp) and rearrangement (Kr) rates of SP, H and the mixed systems with PS.The parameters of adsorption depended of the protein size; concentration and PS used.Kd and Kp showed protein or PS effects depended the concentration of protein; however, when Kr was analyzed, the effect of hydrolysis and PS added showed to have  a big importance in the films properties, which represents the behavior at long times of adsorption. 

Author Biography

Karina Dafne Martinez, University of Buenos Aires
Buenos Aires, Capital Federal


Baeza, R. I., Carrera, C., Pilosof, A. M. R., & Rodrıguez Patino, J. M. (2004). Interfacial and foaming properties of propylenglycol alginates. Effect of degree of esterification and molecular weight. Colloids & Surfaces B: Biointerfaces, 36, 139–145.

Benjamins, J. (2000). Static and dynamic properties of protein adsorbed at liquid interfaces. Ph.D. Thesis, Wageningen University.

Carp, D. J., Wagner, G. B., Bartholomai, G. B. & Pilosof, A. M. R. (1997). Rheological method for kinetics of drainage and disproportionation of soy proteins foams. Journal of Food Science, 62, 1105–1109.

Carrera, C., Rodríguez Niño, M.R., Molina, S.E., Añón, M.C., & Rodríguez Patino, J.M. (2004). Soy globulin spread films at the air-water interface. Food Hydrocolloids, 18, 335-347.

Church, F.C., Swaisgood, H.E, Porter, D.H., & Catignani, G.L. (1983). Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. Journal Dairy Science, 66, 1219-1227.

Damodaran, S., & Song, K. B. (1988). Kinetics of adsorption of protein at interfaces:
role of protein conformation in diffusional adsorption. Biochimica et Biophysica Acta, 954, 253–264.

Dickinson, E. (2003). Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocolloids, 17, 25–40.

Graham, D.E., & Phillips, M.C. (1979). Proteins at liquid interfaces II. Adsorption isotherms, Journal of Colloid Interface Science, 70, 415-426.

Horne, D.S. & Rodriguez Patino, J.M. (2003). Adsorbed biopolymers: behavior in food applications. In Biopolymers at interfaces M. Malmsten (Ed), (pp. 857-900). New York. Dekker

Kato, A., & Nakai, S. (1980). Hydrophobicity determined by a fluorescence probe methods and its correlation with surface properties of proteins. Biochimica et Biophysica Acta, 624, 13-20.

Kim, S. H. & Kinsella, J. E. (1987a). Surface active properties of food proteins: Effects of reduction of disulfide bonds on film properties and foams stability of glycinin. Journal of Food Science, 52, 128–131.

Kim, S. H. & Kinsella, J. E. (1987b). Surface active properties of proteins: Effects of progressive succinylation on film properties and foams stability of glycinin. Journal of Food Science, 52, 1341–1352.

Kinsella, J.E. (1979). Functional properties of soy proteins. Journal of the American Oil Chemist´s Society, 56, 242-258.

Labourdenne, S., Gaudry-Rolland, N., Letellier, S., Lin, M., Cagna, A., Esposito, G., Verger, R., & Rivière, C. (1994). The oil-drop tensiometer: potential applications for studying the kinetics of (phospho)lipase action. Chemistry and Physics of Lipids, 71(2), 163-173.

Liu, M., Lee,D-S., & Damodaran, S. (1999). Emulsifying properties of acidic subunits of soy 11S globulin. Journal of Agricultural and Food Chemistry, 47, 4970-4975.

Macritchie, F., C.B. Anfinsen, J. T. E., & Frederic, M. R. (1978). Proteins at Interfaces. Advances in Protein Chemistry, Volume 32 (pp. 283-326): Academic Press.

MacRitchie, F. (1990). Chemistry at interfaces. San Diego, CA: Academic Press.

Martin, A. H., Bos, M. A., & van Vliet, T. (2002). Interfacial rheological properties and conformational aspects of soy glycinin at the air/water interface. Food Hydrocolloids, 16(1), 63-71.

Martínez, K.D., Baeza, R.I., Millán, F. & Pilosof, A.M.R. (2005). Effect of limited hydrolysis of sunflower protein on the interactions with polysaccharides in foams. Food Hydrocolloids, 19, 361-369.

Martínez, K.D., Carrera, C., Pizones, V., Rodríguez Patino, J. M., & Pilosof, A.M.R. (2007). Effect of limited hydrolysis of soy protein on the interactions with polysaccharides at the air-water interface. Food Hydrocolloids,21,813-822.

Minones, J., Jr., & Rodrıguez Patino, J.M. (2007). The effect of enzymatic treatment of a sunflower protein isolate on the rate of adsorption at the air–water interface. Journal of Food Engineering, 78, 1001–1009.

Ortiz, S. E. M., Sánchez, C. C., Rodríguez Niño, M. R., Añon, M. C., & Rodríguez Patino, J. M. (2003). Structural characterization and surface activity of spread and adsorbed soy globulin films at equilibrium. Colloids and Surfaces B: Biointerfaces, 32(1), 57-67.

Pérez, O. E., Carrera-Sánchez, C., Rodríguez-Patino, J. M., & Pilosof, A. M. R. (2007). Adsorption dynamics and surface activity at equilibrium of whey proteins and hydroxypropyl-methyl-cellulose mixtures at the air-water interface. Food Hydrocolloids, 21(5-6), 794-803.

Rodriguez Niño, M.R. & Rodríguez Patino, J.M (2002). Effect of the aqueous phase composition on the adsorption of bovine serum albumin to the air-water interface. Industrial and Engineering Chemistry Research, 41, 1489-1495.

Rodríguez Niño, M.R, Rodríguez Patino, J.M, Carrera, C, Cejudo, M. & Navarro, J.M. (2003). Physicochemical characteristics of food lipids and proteins at fluid-fluid interfaces. Chemical Engineering Comm., 190, 15-47.

Rodrıguez Patino, J. M., & Rodrıguez Nino, M. R. (1999). Interfacial characteristics offood emulsifiers (proteins and lipids) at the air–water interface. Colloids & Surfaces B: Biointerfaces, 15, 235–252.

Rodrıguez Patino, J. M., Rodriguez Nino, M. R., & Carrera Sanchez, C. (1999). Adsorption of whey protein isolate at the oil–water interface as a function of processing conditions: A rheokinetic study. Journal of Agricultural and Food Chemistry, 47, 3640–3648.

Rodriguez Patino, J. M., Molina Ortiz, S. E., Carrera Sánchez, C., RodrIguez Niño, M. R., & Añón, M. C. (2003). Dynamic properties of soy globulin adsorbed films at the air-water interface. Journal of Colloid and Interface Science, 268(1), 50-57.

Utsumi, S., Matsumura,Y., & Mori, T. (1997). Structure function relationships of soy protein. In S. Damodaran & A. Paraf (Eds). Food Proteins and their application (pp-257-291). New York: Dekker.

Wagner, J.R., & Guéguen, J. (1999). Surface Functional Properties of Native, Acid-Treated, and Reduced Soy Glycinin. 1. Foaming Properties. Journal of Agricultural and Food Chemistry, 47, 2173-2187.

Ward, A., & Tordai, L. (1946). Time dependence of boundary tensions of solutions. I. The role of diffusion in time effects. Journal of Chemical Physics, 14, 353–361.

Xu, S., & Damodaran, S. (1994). Kinetics of adsorption of protein at the air–water interface from a binary mixture. Langmuir, 10, 472–480.

Yu , M.-A., & Damodaran, S. (1991). Kinetics of destabilization of soy proteins foams, Journal of Agricultural and Food Chemistry, 39, 1563-1567.

Zylberman,V., & Pilosof, A.M.R.(2002). Relationship between the Glass Transition, Molecular Structure and Functional Stability of Hydrolyzed Soy Proteins. In H. Levine (Ed.), Amorphous Food and Pharmaceutical Systems (pp. 158-168). Royal Society of Chemistry.