Preliminary phytochemical and in-vitro pharmacological evaluation of whole plant extracts of Ziziphus nummularia Burn. f.

  • Manjusha Choudhary Kurukshetra University, Kurukshetra
  • Ojashvi Sharma Student
  • Nitesh Choudhary Assistant Professor
  • Vikaas Budhwaar Assistant Professor
  • Vineet Mittal Student
Keywords: Ziziphus nummularia, Immunostimulating, Neutrophils, Chemotaxis, Anti-inflammatory.

Abstract

The aim of the present study is to evaluate the in-vitro pharmacological activities of various fractions of hydroalcoholic extracts of Ziziphus nummularia Burn. f. The anti-inflammatory activity was performed by heat induced haemolysis, membrane stabilization and protein denaturation inhibition assay. Diclofenac sodium was used as standard drug. The immunomodulatory activity was carried out on human neutrophils by assessing nitrobluetetrazolium test, neutrophil locomotion and chemotaxis.  All the extracts were tested at concentrations viz. 100, 250, 500 and 1000 μg/ml. The various extracts showed significant activity on in vitro human neutrophils and protein denaturation which are comparable to standard and control at different concentration indicating the possible immune-stimulating, anti-inflammatory and antimicrobial effect.

Author Biographies

Manjusha Choudhary, Kurukshetra University, Kurukshetra
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India
Ojashvi Sharma, Student
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India
Nitesh Choudhary, Assistant Professor
R. P. Inderprastha Institute of Technology, Karnal-132001, Haryana, India
Vikaas Budhwaar, Assistant Professor
Department of Pharmaceutical Sciences, Maharishi Dyanand University, Rohtak-124001,    Haryana, India
Vineet Mittal, Student
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India

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Published
2015-10-23
Section
Articles