Preliminary phytochemical and in-vitro pharmacological evaluation of whole plant extracts of Ziziphus nummularia Burn. f.

  • Manjusha Choudhary Kurukshetra University, Kurukshetra
  • Ojashvi Sharma Student
  • Nitesh Choudhary Assistant Professor
  • Vikaas Budhwaar Assistant Professor
  • Vineet Mittal Student
Keywords: Ziziphus nummularia, Immunostimulating, Neutrophils, Chemotaxis, Anti-inflammatory.


The aim of the present study is to evaluate the in-vitro pharmacological activities of various fractions of hydroalcoholic extracts of Ziziphus nummularia Burn. f. The anti-inflammatory activity was performed by heat induced haemolysis, membrane stabilization and protein denaturation inhibition assay. Diclofenac sodium was used as standard drug. The immunomodulatory activity was carried out on human neutrophils by assessing nitrobluetetrazolium test, neutrophil locomotion and chemotaxis.  All the extracts were tested at concentrations viz. 100, 250, 500 and 1000 μg/ml. The various extracts showed significant activity on in vitro human neutrophils and protein denaturation which are comparable to standard and control at different concentration indicating the possible immune-stimulating, anti-inflammatory and antimicrobial effect.

Author Biographies

Manjusha Choudhary, Kurukshetra University, Kurukshetra
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India
Ojashvi Sharma, Student
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India
Nitesh Choudhary, Assistant Professor
R. P. Inderprastha Institute of Technology, Karnal-132001, Haryana, India
Vikaas Budhwaar, Assistant Professor
Department of Pharmaceutical Sciences, Maharishi Dyanand University, Rohtak-124001,    Haryana, India
Vineet Mittal, Student
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136118, Haryana, India


1. Dashputre, N.L., Naikwade, N.S. Immunomodulatory activity of Abutilon Indicum linn in albino mice. International Journal of Pharma Sciences and Research. 2010; 1(3): 178-184.
2. Nicoara, E., Crisan, A. Possibilities to modulate the immune response during infections. Timisoara Medical Journal. 2003; 53(4): 303-307.
3. Patwardhan, B., Kalbag, D., Patki, P.S., Nagsampagi, B.A. Search of immunomodulatory agents. Indian Drugs. 1990; 28:56-63.
4. Rao, C.S., Raju, C., Gopumadhavan, S., Chauhan, B.L, et al. Immunotherapeutic modification by an Ayurvedic formulation Septilin. Indian Journal of Experimental Biology. 1994; 32:553-8.
5. Gerard, J. Tortora., Sandra, Reynolds., eds. Principles of Anatomy and Physiology. Harper Collins College Publishers 7th edition, 1993; 695
6. Murphy, M.C. Plant products as antimicrobial agents. Clinical Microbiology Review. 1999;12:564-572.
7. Berahou, A.A., Auhmani, A., Fdil, N., Benharref, A., Jana, M., Gadhi, C.A. Antibacterial activity of Quercus ilex bark's extracts. Journal of Ethnopharmacology. 2007:112:426-429.
8. Khandelwal, K.R. Practical Pharmacognosy, Techniques and experiments 12th Edn. Nirali Prakashan, Pune India.2004;157.
9. Kokate, C.K. Practical Pharmacognosy. 4th Edn. Vallabh Prakashan: New Delhi. 1994:120-124.
10. Perez, C., Paul, M., Bazerque, P. An antibiotic assay by the agar well diffusion method. Acta Bio Med Exp, 1990;15:113-115
11. Gandhidasan, R., Thamaraichelvan, A., Baburaj, S. Anti-inflammatory action of Lannea coromandelica by HRBC membrane stabilization. Fitoterapia. 1991;62(1); 81- 83.
12. Samnelsson, G., Kyeremater, G., Farah, M.H. Journal of Ethnopharmacology. 1985, 14: 193.
13. Grant, N.H., Alburn, H.E., Kryzanauskas, C. Stabilization of serum albumin by anti-inflammatory drugs. Biochemistry and Pharmacology. 1970;19: 715 -722.
14. Wilkinson PC. Neutrophil leucocyte function test, Tech in Clin Immunol, 2nd edition, Edited by Thomson RA, Blackwell Scientific Publication, Oxford, London, 1981 :273-93.
15. Gooi, C. A practical approach. Clinical Immunology. 1990:51-78.
16. Thakur, M., Bhargava, S. Dixit, V.K. Immunomodulatory activity of Chlorophytum borivilianum Sant. F. eCAM, doi 2006:1093.
17. Chatterjee, S., Das, S.N. Antiarthritic and antiinflammatiory effects of a polyherbal drug (Ease): its mechanism of action. Indian Journal of Pharmacology. 1996; 28: 116-119.
18. Mizushima, Y. Screening test for anti-rheumatic drugs. Lancet. 1966;2: 443.