Advances in Chemistry and Biochemistry Sciences <p style="text-align: justify;"><span style="font-family: georgia,palatino; font-size: 12pt;"><span style="color: #008000;"><strong>Advances in Chemistry and Biochemistry Sciences (ACBS)</strong></span> journal (ISSN: 2423-6748) is an international peer-reviewed journal that publishes original and high-quality research papers in all areas of <strong>Chemistry and Biochemistry Sciences</strong>. As an important academic exchange platform, scientists and researchers can know the most up-to-date academic trends and seek valuable primary sources for reference. <br /></span></p> en-US Advances in Chemistry and Biochemistry Sciences 2423-6748 Preliminary phytochemical and in-vitro pharmacological evaluation of whole plant extracts of Ziziphus nummularia Burn. f. <p>The aim of the present study is to evaluate the<em> in-vitro</em> pharmacological activities of various fractions of hydroalcoholic extracts of <em>Ziziphus nummularia </em>Burn. f. The anti-inflammatory activity was performed by heat induced haemolysis, membrane stabilization and protein denaturation inhibition assay. Diclofenac sodium was used as standard drug. The immunomodulatory activity was carried out on human neutrophils by assessing nitrobluetetrazolium test, neutrophil locomotion and chemotaxis.  All the extracts were tested at concentrations viz. 100, 250, 500 and 1000 μg/ml. The various extracts showed significant activity on <em>in vitro </em>human neutrophils and protein denaturation which are comparable to standard and control at different concentration indicating the possible immune-stimulating, anti-inflammatory and antimicrobial effect.</p> Manjusha Choudhary Ojashvi Sharma Nitesh Choudhary Vikaas Budhwaar Vineet Mittal ##submission.copyrightStatement## 2015-10-23 2015-10-23 3 1 Vitamin and Mineral content of Ageratum Conyzoides (goat weed) <strong>Abstract: </strong>The Leaf extract of <em>Ageratum conyzoides </em>(goat weed) was analyzed for its Vitamins and Mineral content. The result revealed that the plant contains the vitamins: ascorbic acid (33.36mg/100g), thiamin (0.22mg/100g), riboflavin (0.16mg/100g), niacin (0.07mg/100g) and tocopherol (0.61mg/100g). The mineral concentrations were measured at 5.75mg/100g Zinc, 2.52mg/100g Iron, 0.51mg/100g Calcium, 0.23mg/100g Phosphorus, and 0.11mg/100g Magnesium. The heavy metal Cadmium (Cd) and lead (Pb) were not detected in the sample. The concentration of these vitamins and minerals in <em>A. conyzoides </em>can be attributed to some of its identified properties such as anti-inflammatory, analgesic, anti-oxidant, anti-allergic, and therapeutic activities that supports its use in herbal medicine Ekeoma Malvina Nwankpa ##submission.copyrightStatement## 2015-09-01 2015-09-01 3 1 Quantitative determination and identification of phenolic compounds of three Tunisian legumes: Vicia faba, Lens culinaris and Phaseolus vulgaris <p>The polyphenol content and chromatographic profiles of three legumes extracts of <em>Vicia faba </em>L., <em>Lens culinaris </em>L<em>.</em> and <em>Phaseolus vulgaris</em> were found and compared<em>.</em> In addition, the total phenol content (TPC) in the cited legumes was measured by colorimetric method while the identification was carried out by chromatography LC-ESI-MS technique. The results of TPC, determined by the Folin-Ciocalteu reagent, had the maximum value in the acidified methanol legume extracts of <em>P. vulgaris</em> (3.201± 0.012 mg EAG/g), <em>L. culinaris</em> (2.591 ± 0.02 mg EAG/g) and <em>V. faba</em> (0.991 ± 0.01 mg EAG/g). Fragmentation behavior of polyphenol compounds were investigated using ion trap mass spectrometry in negative electrospray ionization. The MS, MS<sup>n</sup> of polyphenols allowed structural characterization of these compounds. Based on the obtained chromatograms, six phenolic compounds were identified in <em>L. culinaris</em> extract as catechin glucoside, catechin gallate, isomer 1 kaempferol tetraglycoside, isomer 2 kaempferol tetraglycoside, kaempferol-3-robinoside-7-rhamnoside and Kaempferol-3-glycoside. In <em>P. vulgaris</em> extract five compounds were identified: methyl catechin gallate, Myricetin-3-pentoside, Kaempferol 3-o-glycoside, Apigenin glycoside and Kaempferol hexose malic acid. Finally, <em>V. faba</em> extract contains also five compounds: catechin gallate, quercetin arabinoside, epicatechin glucoside, methyl epicatechin gallate and kaempferol glucoside sulfate.</p> JOURNI MOHAMED Hédi Hammouda Malika Trabelsi-Ayadi Jamila Kalthoum Chérif ##submission.copyrightStatement## 2015-08-13 2015-08-13 3 1 Nutritional Science: Evolving approaches to Molecular Homeostasis Consumption of whole organisms by one another forms natural characteristic of food chains and apparent determinant of consistency of basic molecular constitution of live beings through the evolutionary sojourn. Progress in nutrition science appears best in consistency to nature’s health code in whole dietetic patterns, programmed for molecular homeostasis. Food production, consumption and utilization have been changing, posing challenges to address disturbances in homeostasis. With simple beginning of defining standard daily intakes, the task in nutrition science progressively gets complex. Impact of disturbed homeostasis is neither transient nor isolated as revealed by metabolic and genomic studies. It may cover within its fold the vulnerabilities from host-pathogen interactions to genesis of variety of chronic diseases and disability. Multidisciplinary advances in science including recent surge in genomics redefine approaches to address perspectives of molecular homeostasis. The very science of human nutrition however may sail to aspired destinations through disciplined adherence to holistic pursuits with new and traditional means and simultaneous awareness to environmental and social facts besides biomedicine. Garima Pandey abhay kumar pandey SS pandey BL Pandey ##submission.copyrightStatement## 2015-06-28 2015-06-28 3 1 Phenolic composition and total antioxidant capacity of the fresh and commercial Tunisian apple juice <p>Six varieties of the fresh Tunisian apple juice and three varieties of the commercial juice were studied on the basis of their detailed polyphenolic profile. The separation and identification of the compounds were carried out by High performance Liquid Chromatography on Reversed Phase (RP-HPLC). Thiolysis coupled to RP-HPLC was used to determinate the nature, the proportion of the constitutive units and the concentration of procyanidins. Moreover, it gave access to the calculation of the average degree of polymerization (DPn flavan-3-ols/procyanidins). The structural analysis of some phenolic compounds was carried out by ion trap mass spectrometer equipped with an electrospray source of ionization in negative mode (LC-ESI-MS).</p>The commercial apple juice contains only four phenolic compounds, with weak concentrations compared to the fresh juice. Lastly, the estimate of the total antioxidant capacity was determined by the FRAP method. The values of the antioxidant capacity in the case of the fresh juices oscillate between 1758 and 3950 µM. In the case of the commercial juice, the obtained results prove that these drinks contain weak capacities of antioxidants compared to the fresh juice. Hammouda Hédi Mohamed Journi Malika Trabelsi-Ayadi Jamila-Kalthoum Chérif ##submission.copyrightStatement## 2015-03-26 2015-03-26 3 1