Polymorphism of manganese superoxide dismutase gene in blighted ovum
Keywords: Polymorphism, blighted ovum, RFLP, abortion
AbstractAbstract Introduction: Blighted ovum is among the most common reasons of abortion during the first three months of pregnancy. Manganese superoxide dismutase (MnSOD) is an important anti-oxidant enzyme in human immune system. It is located on 6q25 chromosome and acts on mitochondrial matrix. About 90% of produced ROS in human cells are removed by MnSOD. In the case of mutation or inactivity of this enzyme, mitochondrial and nuclear DNA will severely be destructed. The most common polymorphism of its gene is Val16Ala. The purpose of current study was investigating a possible mutation in women who had to abort during first two months of pregnancy because of blighted ovum. Study design: In a case-control study, 34 patients and 34 healthy subjects were entered. Genome DNA was extracted from saliva samples and its genotype was determined using Tetra Primer ARMS-PCR technique. Statistical analysis was carried out by Madcalc (Version 12/1) software. Results: Respective frequency of TT, CT and CC genotypes were 47%, 50% and 3% in patients and 22%, 17% and 61% in control group. Statistical analysis revealed a significant relationship between Val16Ala polymorphism of MnSOD gene and blighted ovum (P = 0.0001). Moreover, allelic frequencies in patients (C=27 and T=73) and control group (C=71 and T=29). (P=0.003, 95% CI = 0.0018, OR = 0.0168) were highly related. Conclusion: Based on the results obtained from our experiments, it is concluded that a significant relationship exists between Val16Ala polymorphism of MnSOD gene and blighted ovum.
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